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The qPCR primers have a little bit decreased but adequately comparable protection to the domain Microbes in comparison to the primers for NGS. The protection of each qPCR and NGS primer sets is very similar for your 4 taxa picked for taxon-unique qPCR (S1B Table), which supplies responsible validation of the explained system Within this research. For upcoming enhancement on the herein offered tactic, a qPCR assay using the exact same primer pair as for NGS may be optimized and validated.
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